Hallet B.

MOLECULAR MECHANISMS OF DNA TRANSPOSITION AND SITE-SPECIFIC RECOMBINATION

GROUP LEADER 

BERNARD HALLET Profile
 
  • 2006-present: Senior Lecturer at UCL
  • 2001-2006: Research associate at the FNRS, Lecturer at UCL
  • 1998-2001: Senior research fellow at the FNRS
  • 1994-1998: Postdoctoral training at the University of Oxford, UK (Biochemistry Dpt, Prof. D.J. Sherratt)
  • 1988-1993: PhD in bacterial genetics at UCL (Biology Dpt, Prof. J. Delcour)
  • 1984-1988:Master degree in Biology at UCL

Fields of expertise: 

  • Molecular and bacterial genetics
  • Biochemistry of protein-DNA interactions
  • Mechanisms of DNA recombination and transposition
  • Genome plasticity and Bacterial cell cycle

 

Biochemistry, Biophysics, and 
Genetics of Microorganisms (BBGM)

 

Bt. Carnoy (b.212)
5 Croix du Sud (Box 6)
B-1348 Louvain-la-Neuve-Belgium

 

 

 

 

Email :
Tel.: +32 10 47 91 60
Fax.: +32 10 47 31 09 

 

RESEARCH OVERVIEW

Research in the laboratory aims at investigating molecular mechanisms that mediate specialised DNA rearrangements in bacteria:

  • DNA transposition
  • Site specific-recombination 

These mechanisms play a central role in bacterial adaptive strategies and evolution. They also provide interesting model systems for the understanding of highly controlled DNA transactions occurring between distant sites in the genome.

Current work is focused on the replicative transposition cycle of Tn4430, a Tn3-family transposon from Bacillus thuringiensis.

The aim of the project is to provide a detailed picture describing how the transposition and recombination machineries encoded by this element assemble and function, and how they are regulated within the cell.

Specificities of Tn4430 transposition and site-specific recombination mechanisms are also used to develop new technologies to manipulate DNA molecules in vivo and in vitro.

  

A second research interest in the laboratory is on the mechanisms that link bacterial cell wall biosynthesis to cell morphology and cell cycle. This study is carried out on two morphologically distinct species of Gram-positive bacteria: the rod-shaped Lactobacillus plantarum and the ovoid Coccus Lactococcus lactis. Part of these activities is conducted in co-supervision with Pascal Hols.

More on research projects >

 

STAFF

TEAMS OF B. HALLET, P. SOUMILLION, AND P. HOLS (BBGM) 

 

Staff 2009-2010  
Group leader Bernard Hallet
Research assistant Blandine David
Graduate students (Ph.D.) Michaël Lambin, Damien Dandoy, Emilien Nicolas
Undergraduate students Nicolas Huguet, Samuel Gengler
Technical assistant Deborah Prozzi
Secretary Liliane Demuylder

More on staff >

| 11/03/2014 |