CV / teaching

SHORT CV-PATRICE SOUMILLION

Date of birth: March 23, 1967
Citizenship: Belgian

EDUCATION

1990-1994:

PhD Thesis in Sciences, UCL (Biochemistry unit), Be

1985-1989:

Post-graduate in Chemistry, UCL (Biochemistry unit), Be                                 

 

PROFESSIONAL

2006-present:

Associate professor at UCL

2000-2006:

Part-time Associate professor at UCL

1998-2006:

Research associate at FNRS

1996-1998:

Postodoctal Researcher at FNRS

1995-1996:

Posdoctoral Researcher at Pennsylvania State University, Prof. S.J. Benkovic, USA                        

1994-1995:

Postdoctoral Researcher at UCL (Biochemistry unit)

1989-1993: Research Fellow at FNRS

 

UCL TEACHING (2009-2010)

CHM1271:

Elements of biochemistry  

CHM1242:

Bio-organic chemistry

 CHM2170: Introduction to protein biotechnology 
BIO1322:

Integrated practicals of biochemistry and molecular biology

CHIM2383: Enzymology and biotechnology II
BBMC2101: Structural and functional biochemistry 
BBMC2105: Protein engineering in enzymology
CEMO3005:

Proteins and biopolymers

 

FIELDS OF EXPERTISE 

  • Enzymology
  • Phage display
  • Directed evolution of proteins

 

PUBLICATIONS (ARTICLES AND BOOKS)

 

2009

  • LABARBE-URBACH Carole, EVRARD Christine, PUDZAITIS Vaidas, FASTREZ Jacques, SOUMILLION Patrice, DECLERCQ Jean-Paul, Structure of PBP-A from thermosynechococcus elongatus, a penicillin-binding protein closely related to class A beta-lactamases, J. Mol. Biol., 386, 2009, p. 109-120.

2008

  • LABARBE-URBACH Carole, FASTREZ Jacques, SOUMILLION Patrice, A new family of cyanobacterial penicillin-binding proteins. A missing link in the evolution of class A beta-lactamases, J. Biol. Chem., 283, 2008, p. 32516-32526.  

2006 

  • FERNANDEZ-GACIO A, CODINA-BARRIOS A, FASTREZ J, RIANT O, SOUMILLION P, Transforming carbonic anhydrase into epoxide synthase by metal exchange, ChemBioChem, 7, 2006, 1013-1016.
  • MATHONET P, BARRIOS-CAMACHO H, SOUMILLION P, FASTREZ J, Selection of allosteric beta-lactamase mutants featuring an activity regulation by transition metal ions, Protein Sci., 15, 2006, 2335-2343.
  • MATHONET P, DEHERVE J, SOUMILLION P, FASTREZ J, Active TEM-1 beta-lactamase mutants with random peptides inserted in three contiguous surface loops, Protein Sci., 15, 2006, 2323-2334.
  • BREDO A, MATHONET P, DESCHUYTENEER G, SOUMILLION P, Gene reconstitution using high efficency homologous recombination between a bacteriophage fd and a plasmid, J. Biotech., 126, 2006, 286-290.

2004

  • SOUMILLION P, LEGENDRE D, FASTREZ J, Chapter 17 : Enzyme engineering by phage display, In: Enzyme functionality, desing, engineering and screening, A. Svendsen ed., New York, USA, Marcel Dekker, 2004, 391-412.
  • SOUMILLION P, Chapter 6 : Selection of phage-displayed enzymes, In: Evolutionary methods in biotechnology, clever tricks for directed evolution, S. Brakmann & A. Schwienhorst ed., Weinheim, Germany, Wiley-VCH Verlag GmbH, 2004, 47-64.
  • EVRARD C, CAPRON A, MARCHAND C, CLIPPE A, WATTIEZ R, SOUMILLION P, KNOOPS B, DECLERCQ J-P, Crystal Structure of a Dimeric Oxidized form of Human Peroxiredoxin 5, J. Mol. Biol., 337, 2004, 1079-1090.
  • HANIQUE S, COLOMBO M, GOORMAGHTIGH E, SOUMILLION P, FRÈRE J, JORIS B, Evidence of an intra molecular interaction between the two domains of the BlaR1 penicillin receptor during the signal transduction, J. Biol. Chem., 279, 2004, 14264-14272.

 2002

  • LEGENDRE D, FASTREZ J, SOUMILLION P, Genetically engineered enzymes as new tools for diagnostics, Bioforum International, 6, 2002, 185-187.
  • SOUMILLION P, FASTREZ J, Investigation of phage display for the directed evolution of enzymes, In: Directed molecular evolution of proteins, Brakmann S. and Johnsson K. ed., Wiley-VCH, 2002, 79-110.
  • LEGENDRE D, VUCIC B, HOUGARDY V, GIRBOUX A-L, HENRIOUL C, VAN HAUTE J, SOUMILLION P, FASTREZ J, TEM-1 beta-lactamase as a scaffold for protein recognition and assay, Protein Sci., 11, 2002, 1506-1518.

2001

  • LEGENDRE D, SOUMILLION P, FASTREZ J, Method for the selective survival or selective growth of a target cell by the use of a conjugate, its use in therapeutics and/or diagnostics and the preparation of said conjugate (brevet), PCT Int. Appl., WO 01/97854, 2001.
  • SOUMILLION P, FASTREZ J, Novel concepts for selection of catalytic activity, Curr. Opin. Biotech., 12, 2001, 387-394.
  • PONSARD I, GALLENI M, SOUMILLION P, FASTREZ J, Selection of metalloenzymes by catalytic activity using phage display and catalytic elution, ChemBioChem., 2, 2001, 253-259.

1999

  • LEGENDRE D, SOUMILLION P, FASTREZ J, Engineering a regulatable enzyme for homogeneous immunoassays, Nat. Biotechnol., 17, 1, 1999, 67-72.
  • EVRARD C, FASTREZ J, SOUMILLION P, Histidine modification and mutagenesis point to the involvement of a large conformational change in the mechanism of action of phage lambda lysozyme, FEBS Lett., 460, 3, 1999, 442-446.
  • ALLEY S, SHIER V, ABEL-SANTOS E, SEXTON D, SOUMILLION P, BENKOVIC S, Sliding clamp of the bacteriophage T4 polymerase has open and closed subunit interfaces in solution, Biochemistry, 38, 1999, 7696-7709. 
  • ALLEY S, JONES A, SOUMILLION P, BENKOVIC S, The carboxyl terminus of the bacteriophage T4 DNA polymerase contacts its sliding clamp at the subunit interface, J. Biol. Chem., 274, 1999, 24485-24489.

1998

  • LEGENDRE D, SOUMILLION P, FASTREZ J, Chimeric target molecules having regulatable activity (Brevet), PCT Int. Appl., WO 9823731, 1998.
  • SOUMILLION P, SEXTON D, BENKOVIC S, Clamp subunit dissociation dictates bacteriophage T4 DNA polymerase holoenzyme disassembly, Biochemistry, 37, 1998, 1819-1827.
  • SOUMILLION P, FASTREZ J, Incorporation of 1,2,4-triazole-3-alanine into a mutant of phage lambda lysozyme containing a single histidine, Protein Eng., 11, 3, 1998, 213-217.

1997

  • VANWETSWINKEL S, AVALLE B, DE CONINCK I, MARCHAND-BRYNAERT J, SOUMILLION P, FASTREZ J, Phage display of bêta-lactamase: selection schemes for catalytic efficiency and turnover, In: Display Technologies: Novel targets and strategies, P.Guttry ed., Southborough, MA (USA), IBC Librariy Series, 1997, 347-365, Chap. 5.1.

1996

  • BERDIS A, SOUMILLION P, BENKOVIC S, The carboxyl terminus of the bacteriophage T4 DNA polymerase is required for holoenzyme formation, Proc. Natl Acad. Sci. USA, 93, 1996, 12822-12827.

1995

  • SOUMILLION P, JESPERS L, VERVOORT J, FASTREZ J, Biosynthetic incorporation of 7-azatryptophan into the phage lambda lysozyme: estimation of trytophan accessibility, effect on enzymatic activity and protein stability, Protein Eng., 8, 1995, 451-456.
  • SOUMILLION P, SARTIAUX P, BOUCHET M, MARCHAND J, FASTREZ J, Enzyme Engineering by in vitro Selection using Genetic and Organic Tools, In: Organic Reactivity : Physical and Biological Aspects, B.T. Golding, R.J. Griffin, H. Maskill ed., 1995, 149-160, The Royal Society of Chemistry, 1995, Special publication, 148.

1994

  • SOUMILLION P, Etude enzymologique du lysozyme du bactériophage lambda,Thèse de Doctorat en Sciences Chimiques défendue en 1994, Faculté des Sciences de l'UCL, 1994.
  • SOUMILLION P, JESPERS L, BOUCHET M, MARCHAND J, SARTIAUX P, FASTREZ J, Phage Display of Enzymes and in vitro Selection for Catalytic Activity, Appl. Biochem. Biotechnol., 47, 1994, 175-190.
  • SOUMILLION P, JESPERS L, BOUCHET M, MARCHAND J, WINTER G, FASTREZ J, Selection of bêta-lactamase on filamentous bacteriophage by catalytic activity, J. Mol. Biol., 237, 1994, 415-422.

1992

  • SOUMILLION P, FASTREZ J, A large decrease in heat-shock-induced proteolysis after tryptophan starvation leads to increased expression of phage lambda lysozyme cloned in Escherichia coli, Biochem. J., 286, 1992, 187-191.
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