Integrated practical work in animal physiology, histology, and biochemistry

Language

French

Prerequisites

BAC2 physiology, histology, and biochemistry course

Main themes

HISTOLOGY
- Preparation of histological sections from paraffin-embedded organs (liver, heart, transverse muscle section, and longitudinal muscle section)
- Staining of sections using PAS staining (to highlight glycogen)
BIOCHEMISTRY
- Measurement of glycogen in the liver and skeletal muscle using a colorimetric technique
PHYSIOLOGY
- Effect of stimulation frequency on muscle contraction
- Effect of stimulation intensity on muscle contraction
- Effect of a pharmacological agent on muscle contraction

Learning outcomes

At the end of this learning unit, the student is able to :
1	Contribution of teaching to the learning outcomes included in the VETE reference framework 1.1.2, 1.2.1 , 1.2.3 , 1.2.4 , 2.1.1 , 2.1.2 , 2.1.3 , 2.1.4 , 2.1.5 , 2.1.6 , 2.1.7 , 3.1.1 , 3.1.2 , 3.1.3 , 4.1.1 , 4.1.2 , 4.1.3 , 6.2.1 , 7.1.1 , 7.1.2 , 7.1.3
2	Essential and/or specific learning outcomes of the course measure muscle contraction using an appropriate device perform histological sections and highlight glycogen in histological preparations measure glycogen in tissues integrate the three disciplines of physiology, histology, and biochemistry to test a scientific hypothesis write a report integrating the results obtained using three different experimental approaches in three different disciplines

Content

The practical work is divided into three parts:
HISTOLOGY
Making histological sections from paraffin-embedded organs (liver, heart, muscle cross-section and muscle sectioned longitudinally
Staining of sections by PAS staining (glycogen detection)
BIOCHEMISTRY
Determination of glycogen in the liver and skeletal muscle by colorimetric technique
PHYSIOLOGY
Effect of stimulation frequency on muscle contraction
Effect of intensity of stimulation on muscle contraction
Effect of a pharmacological agent on muscle contraction

Teaching methods

Practical work supervised by the assistants.

Evaluation methods

Writing a report integrating the three components of the activity.

Online resources

https://moodleucl.uclouvain.be/course/view.php?id=7626

Bibliography

Teaching materials

• Syllabus avec les instructions pour les travaux pratiques

Faculty or entity

> VETE