Flow Cytometry and Cell Sorting Facility (CYTF)
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Picture credit : Hugues Depasse
Technical and scientific expertise
- Consultancy in experimental planning, experimental execution and data interpretation
- Theoretical and practical training on analysers and the MA900 sorter
- FlowJo analysis software training
Main equipment
Flow cytometer analysers
- BD FACSVerse (3L ; FSC/SSC + 8 Fluorescent parameters)
- BD LSR Fortessa (4L-YG ; FSC/SSC + 16 Fluorescent parameters)
- Cytek Aurora (4L-YG ; FSC/SSC-B/SSC-V + full spectrum)
Cell sorters
- BD FACS ARIA III (4L-YG ; FSC/SSC + 16 Fluorescent parameters)
- Sony MA900 (4L ; FSC/SSC + 12 fluorescent parameters)
- Cytek Aurora CS (4L-YG ; FSC/SSC-B/SSC-V + full spectrum)
Single-cell RNAsequencing (scRNAseq)
- 10x Genomics : chromium Platform
- MANTIS® - Microfluidic Liquid Dispenser (Formulatrix) for Smart-seq2
Automated imaging
- 4 automated cell imaging : Molecular Devices, ImageXpress-PICO
Applications
- Simultaneous multiparametric analysis of thousands of cells per second
- Analysis of complex cell populations for phenotypic and functional characteristics
- Physical separation of identified cell populations for downstream characterisation
Services available to
- UCLouvain students and researchers
- Non-UCLouvain parties