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Flow Cytometry and Cell Sorting Facility (CYTF)

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Picture credit : Hugues Depasse

Technical and scientific expertise

  • Consultancy in experimental planning, experimental execution and data interpretation
  • Theoretical and practical training on analysers and the MA900 sorter
  • FlowJo analysis software training

Main equipment

Flow cytometer analysers

  • BD FACSVerse (3L ; FSC/SSC + 8 Fluorescent parameters)
  • BD LSR Fortessa (4L-YG ; FSC/SSC + 16 Fluorescent parameters)
  • Cytek Aurora (4L-YG ; FSC/SSC-B/SSC-V + full spectrum)

Cell sorters

  • BD FACS ARIA III (4L-YG ; FSC/SSC + 16 Fluorescent parameters)
  • Sony MA900 (4L ; FSC/SSC + 12 fluorescent parameters)
  • Cytek Aurora CS (4L-YG ; FSC/SSC-B/SSC-V + full spectrum)

Single-cell RNAsequencing (scRNAseq) 

  • 10x Genomics : chromium Platform
  • MANTIS® - Microfluidic Liquid Dispenser (Formulatrix) for Smart-seq2

Automated imaging

  • 4 automated cell imaging : Molecular Devices, ImageXpress-PICO

Applications

  • Simultaneous multiparametric analysis of thousands of cells per second
  • Analysis of complex cell populations for phenotypic and functional characteristics
  • Physical separation of identified cell populations for downstream characterisation

Services available to

  • UCLouvain students and researchers
  • Non-UCLouvain parties