Proteomic & protein analysis by mass spectrometry (MASSPROT)

Picture credit : Yvan Flamant - MASSPROT Woluwé

Technical and scientific expertise

Proteomics: scientific and technical advice and support at all stages of sample preparation and mass spectrometry experiments in areas such as:

• identification and expression changes of proteins
• identification and quantification of proteins from complex samples
• localisation of post-translational modifications even in complex samples
• analysis of intact proteins

Bioinformatics: advice and data analysis using appropriate IT tools and software

Main equipment

Mass spectrometry, chromatography, two-dimensional electrophoresis techniques:

• Orbitrap Fusion Lumos Tribrid Mass spectrometer (Thermo Scientific)
• Orbitrap Exploris 240 Mass spectrometer (Thermo Scientific)
• Synapt G2si spectrometer (Waters)
• Acquity MClass nano UPLC System (Waters)
• Ultimate 3000 RSLN nano HPLC System (Thermo Scientific)

Get an overview of the platform’s facilities via a virtual tour.

Applications

• Measurement of intact protein masses for quality control
• Identification of purified proteins on gel
• De novo sequencing of peptides for protein identification
• Quantification of differential protein expression
• Identification of covalent modification sites by peptide fragmentation/dissociation

Services available to

• UCLouvain students and researchers
• Non-UCLouvain parties